Henry V. HuangAssociate ProfessorPhone: (314)-362-2755 FAX: (314)-362-7325 email: huang@wustl.edu
8230 McDonnell Pediatric Research Building |
Our understanding of the molecular biology of Sindbis virus has led to two types of practical applications. First, it has suggested a novel and general approach for designing antiviral drugs, targeting highly conserved elements of the viral genome, including the viral recognition of the cis-acting sequences for initiation of replication and transcription,. It promises to provide broad-spectrum antiviral drugs that are effective against the virus and having minimal impact on host processes, and with very low probabilities of drug-resistance. We are using Sindbis virus as a model system to test this novel approach.
Second, we have developed a series of RNA virus gene expression vectors, derived from Sindbis virus, that give rapid and efficient expression of foreign genes in a wide range of host cells. The Sindbis virus vectors are being used by many research groups to express specific RNAs and proteins in cultured cells and in animals. In our laboratory, we will be using them to express and characterize human developmental gene regulators that are up-regulated during the monocytic differentiation of a human promyelocytic leukemia cell line.
Keywords: Molecular biology and evolution of RNA viruses, RNA virus gene expression vectors. Antiviral drug design.
Present position | |||||||
1992 - | Associate Professor Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri | ||||||
Education | |||||||
1972 | A.B. Biology, Occidental College, Los Angeles, California | ||||||
1977 | Ph.D. Biochemistry, California Institute of Technology, Pasadena, California. Thesis advisor: Dr. William J. Dreyer. | ||||||
Professional Experience and Appointments | |||||||
1982-1992 | Assistant Professor Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri | ||||||
1981-1982 | Senior Research Fellow Division of Biology, California Institute of Technology, with Dr. Leroy Hood | ||||||
1982 | Invited Instructor faculty course on techniques in molecular biology, California State University, Northridge, California. | ||||||
1980-1981 | Senior Research Fellow in Biology and Biomedical Engineering Divisions of Biology, and Engineering and Applied Science, California Institute of Technology, with Drs. Leroy Hood and Derek Fender | ||||||
1979-1980 | Research Fellow in Biology and Biomedical Engineering Divisions of Biology, and Engineering and Applied Science, California Institute of Technology, with Drs. Leroy Hood and Derek Fender | ||||||
1979-1980 | Part time Instructor Division of Biology, California Institute of Technology | ||||||
1977-1979 | American Cancer Society Postdoctoral Fellow Division of Biology, California Institute of Technology, with Dr. Leroy Hood | ||||||
1971-1972 | Member of BUGSYS design team for computer analysis of video images Jet Propulsion Laboratory, California Institute of Technology | ||||||
Honors | |||||||
1970 | Seltzer Award, Occidental College | ||||||
1970-1972 | College Scholar, Occidental College | ||||||
1972 | A.B. Summa Cum Laude, Occidental College | ||||||
1972 | Alpha Mu Gamma, National Honorary Language Fraternity | ||||||
1972 | Phi Beta Kappa | ||||||
1972 | Association for the Advancement of Biomedical Education Award | ||||||
1972-1977 | Danforth Fellow | ||||||
1976-1977 | McCallum Fellowship, California Institute of Technology | ||||||
1977-1979 | American Cancer Society Postdoctoral Fellow | ||||||
1995-1999 | Member, NIH Virology Study Section | ||||||
International Committees | |||||||
1997-present | International Committee on Taxonomy of Viruses. Togaviridae Study Group | ||||||
Editorial Responsibilities | |||||||
1982- | Ad Hoc Reviewer: Journal of Virology, Proceedings of the National Academy of Science, USA, Virus Research, Nucleic Acids Research | ||||||
Professional Society Membership | |||||||
American Association for the Advancement of Science | |||||||
American Society for Microbiology | |||||||
American Society for Virology | |||||||
Genetics Society of America | |||||||
Sigma Xi | |||||||
University Appointments and Committees Thesis committees 1982-present | |||||||
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Other committees | |||||||
1983-5 | Member, Nucleic Acids Preliminary Examination Committee | ||||||
1986 | Chair, Nucleic Acids Preliminary Examination Committee | ||||||
1985-86, 88 | Member, Developmental Biology Preliminary Examination Committee | ||||||
1987 | Chair, Developmental Biology Preliminary Examination Committee | ||||||
1988 | Member, Genetics Preliminary Examination Committees | ||||||
1991 | Chair, Virology Preliminary Examination Committee | ||||||
1983 | Member, Cell and Molecular Biology Programs Admissions Committee | ||||||
1984-87 | Secretary, Cell and Molecular Biology Programs Admissions Committee | ||||||
1988 | Secretary, Cell, Immunology and Molecular Biology Programs Admissions Committee | ||||||
1988-1992 | Member, Medical Scientist Training Program Committee | ||||||
1991 | Medical Scientist Training Program Director Search Committee | ||||||
1990-present | Supervisor, Departmental computing services | ||||||
1986-1988 | Member, Steering Committee of the Molecular Biology: Genetics and Biochemistry Program | ||||||
1988-1991 | Co-coordinator, Molecular Biology: Genetics and Biochemistry Program | ||||||
1991-1992 | Coordinator, Molecular Microbiology & Microbial Pathogenesis Program | ||||||
1991-1992 | Member, Governing Committee of the Programs in Cell and Molecular Biology | ||||||
1990-1991 | Senior Advisory Committee | ||||||
1991 | Co-chairman, Cell Molecular Biology Merger Committee | ||||||
1992 | Member, Programs and Student Affairs Committee | ||||||
1992-1994 | Member, Minority subcommittee, Committee on Admissions, School of Medicine | ||||||
1992-1996 | Member, Recruitment Committee of the Division of Biology and Biomedical Sciences | ||||||
1997 | Information Services Committee, of the Medical School Process Improvement Program | ||||||
1996-2000 | Member, Committee on Admissions, School of Medicine | ||||||
2001-2005 | Member, Institutional Biological and Chemical Safety Committee | ||||||
2005-present | Co-chair, Institutional Biological and Chemical Safety Committee | ||||||
Teaching | |||||||
1983 | Microbiology: Molecular Biology of Prokaryotes | ||||||
1983, 1984 | Foundations in Immunology | ||||||
1983, 1984 | Topics in Immunology | ||||||
1983, 1986 | Regulatory Phenomena in Cell and Molecular Biology | ||||||
1984 | Selected Topics in Developmental Biology: Mechanisms of DNA Recombination and Rearrangement | ||||||
1987 | Advanced Genetics | ||||||
1989 | Practical Applications of Viruses | ||||||
1990-91 | Microbiology | ||||||
1994 | Molecular Virology | ||||||
1995 | Ethics and Research Science | ||||||
1995-1998 | Co-coursemaster, Molecular Virology | ||||||
1997-present | Molecular Microbiology and Pathogenesis | ||||||
1999-2004 | Coursemaster, Molecular Virology | ||||||
1999-2001 | Microbes and Pathogenesis | ||||||
2000-2001 | Discussion group leader, Nucleic acids and Protein Synthesis | ||||||
2004 | Co-coursemaster, Special Topics in Microbial Pathogenesis | ||||||
2002-present | Co-coursemaster, Microbes and Pathogenesis | ||||||
Participation on Training Grants | |||||||
1984-present | Cell and Molecular Biology Training Grant | ||||||
1984-present | Program Physician-Scientist Award | ||||||
1984-present | Infectious Disease Training Grant | ||||||
Peer Review Panels | |||||||
1982-present | Ad hoc and special reviewer / site visit panels, for NIADDK, NIAID, NSF. | ||||||
1995-1999 | Member, Virology study section, NIH. | ||||||
Community Service | |||||||
1989-1997 | Judge, Monsanto-St. Louis Post Dispatch Science Fair, Honors Division. | ||||||
1994-2000 | Technology Committee, Forsyth School | ||||||
1995-2003 | Board of Trustees, Forsyth School |
Sindbis virus gene expression vectors
These are infectious RNA virus vectors with excellent levels of gene expression in a variety of vertebrate and invertebrate cells.
They can be used as expression vectors, vaccine reagents, for non-integrating genetic immunization, etc..
Department of Molecular Microbiology, Box 8230 Washington University School of Medicine St. Louis, MO 63110-1093 USA | Tel 314-362-2755 FAX 314-362-7325 |